rabbit polyclonal anti tnfsf12  (Boster Bio)

Journal: Journal of orthopaedic translation

Article Title: Upregulation of FABP4 induced inflammation in the pathogenesis of chronic tendinopathy.

doi: 10.1016/j.jot.2024.06.007

Figure Lengend Snippet: Fig. 2. Co-localization of FABP4 with inflammatory cytokines in rotator cuff tendinopathy. Photomicrographs showing co-localization of FABP4 with IL-1β or TNF-α in human rotator cuff tendinopathy samples. Scale bar = 50 µm; n = 5/group; white arrows: co-localized signal in tendon cells; red arrows: co-localized signal in blood vessels.

Article Snippet: The sections were stained with goat polyclonal antibodies against mouse/rat FABP4 (1:200) (R&D Systems, Inc., Minneapolis, MN, USA; catalog # AF1443) overnight at 4 ◦C, washed and stained with rabbit polyclonal antibodies against human/mouse/rat IL-1β (1:200) (Origene, catalog # TA321162) or rabbit polyclonal antibodies against human/mouse/rat TNF-α (1:200) (Affinity, OH, USA; catalog # AF7014-100) for 2 h at room temperature.

Techniques:

Journal: Journal of orthopaedic translation

Article Title: Upregulation of FABP4 induced inflammation in the pathogenesis of chronic tendinopathy.

doi: 10.1016/j.jot.2024.06.007

Figure Lengend Snippet: Fig. 4. Upregulation of FABP4 and inflammatory cytokines in mouse CI animal model. Photomicrographs showing the immunohistochemical staining of FABP4, IL-1β, IL-6, TNF-α and IL-10 in representative samples of mouse Achilles tendons at (A) week 2 and week 8 after saline or collagenase injection. (B) Boxplots showing the IOD/mm2 of target markers. n = 5/group; Scale bar = 100 μm (insert 25 μm); red arrow: hypercellular area; green arrow: small, nucleated cells; *: chondrocyte-like cells; CR: calcified region; black arrow: immunopositive cells; **p < 0.01; ***p < 0.001

Article Snippet: The sections were stained with goat polyclonal antibodies against mouse/rat FABP4 (1:200) (R&D Systems, Inc., Minneapolis, MN, USA; catalog # AF1443) overnight at 4 ◦C, washed and stained with rabbit polyclonal antibodies against human/mouse/rat IL-1β (1:200) (Origene, catalog # TA321162) or rabbit polyclonal antibodies against human/mouse/rat TNF-α (1:200) (Affinity, OH, USA; catalog # AF7014-100) for 2 h at room temperature.

Techniques: Animal Model, Immunohistochemical staining, Staining, Saline, Injection

Journal: Cell reports

Article Title: Lactic acid induces transcriptional repression of macrophage inflammatory response via histone acetylation

doi: 10.1016/j.celrep.2024.113746

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal biotinylated anti-mouse TNF-α , eBioscience , Cat# 13-7341-81; RRID: AB_466950.

Techniques: Recombinant, Luciferase, Reporter Assay, Staining, Control, Software

Journal: Antioxidants

Article Title: Geranylgeranylacetone Ameliorates Skin Inflammation by Regulating and Inducing Thioredoxin via the Thioredoxin Redox System

doi: 10.3390/antiox12091701

Figure Lengend Snippet: Topical and oral treatment with GGA inhibited the expression of TNF-α, IL-1β, GM-CSF, and 8-OHdG in a croton oil-induced ICD model. ( A – D ) Immunohistochemistry was performed to detect the effects of GGA on cytokine production and antioxidants. Immunohistochemical staining showed that the expression of TNF-α, IL-1β, GM-CSF, and 8-OHdG was strongly inhibited by GGA; bars 100 μm; n = 5 mice for each group. ( E – H ) The mean of IOD expression of TNF-α, IL-1β, GM-CSF, and 8-OHdG. Values are shown as the mean ± SD (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA with post hoc Tukey’s).

Article Snippet: The tissue specimens were incubated with rabbit anti-mouse TNF-α polyclonal antibody (1:200; Bioss, Beijing, bs-10802R, China); rabbit anti-mouse IL-1β polyclonal antibody (1:200; Bioss bs-0812R; China); rabbit anti-mouse TRX monoclonal antibody (1:1000; Fujirebio, Tokyo, Japan); mouse anti-mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) monoclonal antibody (1:200; Santa Cruz Biotechnology, sc393871, Shanghai, China); rabbit anti-mouse GM-CSF polyclonal antibody (1:200; Bioss, bs3790R, China); rabbit anti-mouse NLRP3 polyclonal antibody (1:500; ABclonal, Wuhan, A21906, China); or mouse anti-mouse Nrf2 monoclonal antibody (1:200; Proteintech, Wuhan, 66504-1-Ig, China) as the primary antibody and incubated overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining

Journal: Antioxidants

Article Title: Geranylgeranylacetone Ameliorates Skin Inflammation by Regulating and Inducing Thioredoxin via the Thioredoxin Redox System

doi: 10.3390/antiox12091701

Figure Lengend Snippet: GGA significantly inhibited the protein expression of TNF-α, IL-1β, GM-CSF, and NLRP3 in PMA-stimulated PAM212 cells. An amount of 1 μM of GGA, 20 μg/mL of rhTRX, and 20 μg/mL of hydrocortisone were added to the medium in the presence of PMA. The protein expression of TNF-α, IL-1β, GM-CSF, and NLRP3 was detected by Western blot at 24 h post-stimulation. The relative expression of cytokines was quantified and normalized to GAPDH. Expression of these proteins was significantly inhibited in the GGA-treated group compared to the PMA-treated group; values are mean ± SD of three independent experiments (* p < 0.05, ** p < 0.01, *** p < 0.001; one-way ANOVA with post hoc Tukey’s).

Article Snippet: The tissue specimens were incubated with rabbit anti-mouse TNF-α polyclonal antibody (1:200; Bioss, Beijing, bs-10802R, China); rabbit anti-mouse IL-1β polyclonal antibody (1:200; Bioss bs-0812R; China); rabbit anti-mouse TRX monoclonal antibody (1:1000; Fujirebio, Tokyo, Japan); mouse anti-mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) monoclonal antibody (1:200; Santa Cruz Biotechnology, sc393871, Shanghai, China); rabbit anti-mouse GM-CSF polyclonal antibody (1:200; Bioss, bs3790R, China); rabbit anti-mouse NLRP3 polyclonal antibody (1:500; ABclonal, Wuhan, A21906, China); or mouse anti-mouse Nrf2 monoclonal antibody (1:200; Proteintech, Wuhan, 66504-1-Ig, China) as the primary antibody and incubated overnight at 4 °C.

Techniques: Expressing, Western Blot

Journal: Nutrients

Article Title: Cordyceps cicadae NTTU 868 Mycelia Fermented with Deep Ocean Water Minerals Prevents D-Galactose-Induced Memory Deficits by Inhibiting Oxidative Inflammatory Factors and Aging-Related Risk Factors

doi: 10.3390/nu15081968

Figure Lengend Snippet: Effects of C. cicadae NTTU 868 fermentation products cultured with various water on the protein expressions of inflammatory factors TNF-α, IL-6, and IL-1β in the cerebral cortex of DG-induced aging rats ( A ) TNF-α, ( B ) IL-6, ( C ) IL-1β, ( D ) COX-2, ( E ) iNOS. Animals, with the exception of the NOR group, were injected daily with D-galactose (500 mg/kg b.w./day). The DCC group received daily administration of C. cicadae fermentation product cultured with DOW (26 mL/kg b.w./day), while the MgCC group received C. cicadae fermentation product cultured with MgCl 2 solution (26 mL/kg b.w./day). The UCC group was administered C. cicadae fermentation product cultured with UPW (26 mL/kg b.w./day), while the HEA group was administered HEA (0.298 µL/kg b.w./day) daily. The data are presented as mean ± standard deviation (n = 7). Mean values within each group with different lowercase letters are significantly different ( p < 0.05).

Article Snippet: The rabbit iNOS antibody (FNab04325), the rabbit anti-rat COX-2 polyclonal antibody (RPD284Ra01), and the rat COX-2 protein (RPD284Ra01) were purchased from Cloud-Clone Corp. (Wuhan, China).The rRabbit anti-mouse TNF-α polyclonal antibody (AB2148P) was purchased from the EMD Millipore Corporation (Temecula, CA, USA).

Techniques: Cell Culture, Injection, Standard Deviation